TABLE 2.

Fatty acid compositions of P. profundum strains as a function of growth pressure and temperature^a

Strain	Culture conditionsb	Mean % (by wt) of fatty acid ± SD										Unsaturation indexc	UFA/SFA ratiod
		12:0	14:0	14:1	iso-16:0	16:0	16:1	12-OH	18:0	18:1	20:5
DB110
Untreated	15°C	1.8 ± 0.9	4.9 ± 1.4	1.2 ± 0.8	9.8 ± 3.1	21.7 ± 2.8	41.3 ± 8.3	3.0 ± 1.2	1.1 ± 0.4	9.9 ± 2.6	5.3 ± 1.1	79 ± 5.1	1.95
	4°C	2.7 ± 1.0	3.3 ± 0.8	2.5 ± 0.5	0.2 ± 0.1	20.2 ± 3.8	48.8 ± 6.8	3.4 ± 1.3	0.2 ± 0.1	10.3 ± 1.7	8.4 ± 1.1	104 ± 6.6	2.65
	0.1 MPa	1.5 ± 0.1	5.0 ± 0.3	0.8 ± 0.1		21.4 ± 1.5	64.1 ± 1.5	0.6 ± 0.1	0.2 ± 0.0	3.6 ± 0.1	2.7 ± 0.1	82 ± 2.4	2.53
	28 MPa	2.2 ± 0.6	1.6 ± 0.7	0.7 ± 0.1		17.5 ± 3.2	48.7 ± 9.6	2.0 ± 0.8	0.1 ± 0.1	16.2 ± 1.6	11.0 ± 0.8	121 ± 3.3	3.57
	50 MPa	1.4 ± 0.8	0.8 ± 0.5	0.5 ± 0.1		12.5 ± 1.2	45.9 ± 7.0	2.3 ± 0.5	0.1 ± 0.1	25.1 ± 4.1	11.4 ± 0.2	129 ± 7.2	5.60
Cerulenin treatede	15°C	4.9 ± 0.8	21.7 ± 2.7	4.5 ± 1.6	0.3 ± 0.3	28.3 ± 4.3	27.3 ± 4.2	3.8 ± 2.1	0.2 ± 0.1	0.3 ± 0.2	8.7 ± 2.6	76 ± 8.9	0.79
	4°C	9.3 ± 0.0	17.9 ± 0.8	9.8 ± 2.3		19.0 ± 0.4	22.4 ± 0.6	8.3 ± 0.1		0.3 ± 0.1	13.0 ± 0.9	98 ± 2.7	0.98
	0.1 MPa	9.5 ± 0.3	24.8 ± 3.2	5.6 ± 1.0		30.0 ± 2.9	10.8 ± 0.8	6.2 ± 0.7	0.2 ± 0.0	0.3 ± 0.0	12.6 ± 1.1	80 ± 1.9	0.45
	28 MPa	16.1 ± 2.3	35.1 ± 3.7	5.3 ± 0.9		17.2 ± 2.0	7.1 ± 1.1	6.3 ± 0.9			12.9 ± 1.2	77 ± 1.7	0.37
EA3
	15°C	8.2 ± 1.3	19.7 ± 2.3	11.7 ± 2.8		21.5 ± 1.4	27.9 ± 2.9	5.0 ± 2.6	3.5 ± 0.5	0.9 ± 0.5	1.6 ± 0.3	49 ± 6.6	0.80
	4°C	9.2 ± 1.2	24.0 ± 0.6	10.9 ± 1.2		22.0 ± 0.7	20.9 ± 1.9	4.3 ± 1.6	0.3 ± 0.1	0.1 ± 0.0	8.3 ± 0.6	73 ± 3.9	0.72
	0.1 MPa	3.9 ± 0.2	19.3 ± 1.7	3.6 ± 0.2		53.4 ± 0.5	11.9 ± 1.1	3.5 ± 0.2	0.8 ± 0.1	0.5 ± 0.1	3.1 ± 0.2	32 ± 2.6	0.25
	28 MPa	3.6 ± 0.1	17.9 ± 0.4	3.1 ± 0.1		53.2 ± 0.1	15.3 ± 0.6	2.7 ± 0.3	1.2 ± 0.1	0.7 ± 0.1	2.3 ± 0.2	31 ± 0.5	0.28
EA2
	15°C	3.5 ± 0.3	16.5 ± 1.2	1.1 ± 0.1		35.9 ± 0.5	10.1 ± 0.3	3.7 ± 2.0	2.6 ± 0.7	0.7 ± 0.1	25.8 ± 1.2	141 ± 6.0	0.64
	4°C	4.9 ± 0.2	24.4 ± 3.2	1.7 ± 0.6		26.9 ± 1.0	7.1 ± 0.5	4.7 ± 1.5	0.4 ± 0.1	0.2 ± 0.1	29.5 ± 1.9	157 ± 3.6	0.68
	0.1 MPa	4.5 ± 0.1	25.2 ± 0.1	0.5 ± 0.5		35.8 ± 2.2	3.9 ± 0.1	4.5 ± 1.1	0.4 ± 0.2	0.2 ± 0.1	24.8 ± 0.9	129 ± 4.5	0.45
	28 MPa	4.3 ± 0.6	15.5 ± 3.1	1.8 ± 0.3		29.7 ± 7.0	9.1 ± 0.1	3.9 ± 0.9	0.2 ± 0.1	0.8 ± 0.4	32.7 ± 4.5	176 ± 21.0	0.89
	50 MPa	4.0 ± 0.1	5.0 ± 0.1	0.4 ± 0.1		35.6 ± 1.3	18.3 ± 1.0	5.1 ± 0.6	0.3 ± 0.0	2.8 ± 0.1	28.3 ± 2.7	163 ± 12.4	1.11
EA10
	15°C	2.5 ± 1.0	6.5 ± 2.2	4.7 ± 0.5	5.0 ± 1.5	16.7 ± 2.8	46.3 ± 3.8	3.3 ± 0.7	1.0 ± 0.2	14.0 ± 1.4		65 ± 3.0	2.43
	4°C	3.0 ± 0.2	3.2 ± 0.7	4.1 ± 0.7	2.1 ± 1.0	10.6 ± 0.9	55.2 ± 3.9	3.9 ± 0.2	0.2 ± 0.1	17.7 ± 3.3		77 ± 1.3	4.53
	0.1 MPa	3.1 ± 0.1	3.7 ± 0.3	2.2 ± 0.3		20.4 ± 0.5	52.9 ± 1.0	4.6 ± 0.4	0.4 ± 0.1	12.7 ± 0.1		68 ± 0.8	2.46
	28 MPa	2.8 ± 0.2	1.4 ± 0.2	1.6 ± 0.0		12.2 ± 2.0	52.3 ± 0.3	4.2 ± 0.1	0.1 ± 0.1	25.4 ± 2.6		79 ± 2.4	4.81
	50 MPa	2.6 ± 1.0	1.6 ± 0.3	1.1 ± 0.2		13.5 ± 0.3	51.6 ± 3.6	5.5 ± 1.2	0.1 ± 0.1	24.0 ± 3.8		77 ± 0.2	4.31

^aData represent values derived from triplicate samples harvested in the late exponential phase of growth. 

^b15 and 4°C cultures were grown aerobically in 2216 marine medium at 0.1 MPa; 0.1-, 28-, and 50-MPa cultures were grown at 9°C in 2216 marine medium containing 22 mM glucose buffered with 100 mM HEPES. 

^cUnsaturation index was calculated as the sum of the mean percentages (by weight) of the UFA species multiplied by the number of double bonds (12). 

^dUFAs, 14:1, 16:1, 18:1, and 20:5; SFAs, 12:0, 14:0, 16:0, and 18:0. 

^eCerulenin was used at 12 μg/ml.