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. 2022 May 21;13(5):486. doi: 10.1038/s41419-022-04928-0

Fig. 8. ADAM15 affects the EGFR signalling pathway through integrin α3/α6.

Fig. 8

A Control and ADAM15-overexpressing stable A549 cells were treated with the indicated siRNA (si-α3/α6 compared with si-NC) followed by western blotting. β-actin was used as a loading control. B, C Control and ADAM15-overexpressing stable A549 cells were treated with the indicated siRNA (si-α3//α6 compared with si-NC), followed by CCK8 and Transwell assays. D Co-immunoprecipitation of ADAM15 and integrin α3/α6 is shown. Protein was immunoprecipitated and detected from parental A549 and H1299 cells using a specific monoclonal antibody. E Co-immunoprecipitation of ADAM15 and integrins were shown, protein were immunoprecipitation from lysates of control and CD151 knockdown H1299 cells using a specific monoclonal antibody. F The Flag-tagged ADAM15 vector and HA-tagged CD151 vector were cotransfected into A549 and H1299 cells for 48 h. The protein were immunoprecipitated using agarose beads with anti-Flag antibody. *P < 0.05; **P < 0.01; ***P < 0.001. G Schematic illustration of the functional roles of ADAM15, CD151 and integrin complexes in NSCLC.