Figure 5.

PJA1 reverses the effect of OTUB2 knockdown on the proliferation and migration of HCC cells. Huh7 and MHCC-97h cells were transfected with sh-OTUB2-1, PJA1 overexpression vectors (PJA1), or both. (a) The transfection efficiency of PJA1 was detected by Western blot (n = 3). After 48 h transfection, cell proliferation was examined by (b) CCK-8 (n = 3) and (c) colony formation (n = 3) assays; cell migration was evaluated by (d) wound healing (n = 3) and (e) transwell (n = 3) assays. Scale bar, 100 μm. Quantitative data are presented as mean ± SD of three replicates. *p < 0.05 and **p < 0.01, compared with the NC group; ^#p < 0.05, compared with the sh-OTUB2-1 group