Figure 6.

PJA1 silencing blocks the effect of OTUB2 overexpression on proliferation and migration of HCC cells. Huh7 and MHCC-97h cells were transfected with OTUB2 OE, short hairpin RNAs against PJA1 (sh-PJA1), or both. (a) The transfection efficiency of sh-PJA1-1 and sh-PJA1-2 was detected by Western blot (n = 3), and sh-PJA1-1 was selected for the subsequent experiment. After 48 h transfection, cell proliferation was examined by (b) colony formation (n = 3) and (c) CCK-8 (n = 3) assays; cell migration was evaluated by (d) wound healing (n = 3) and (e) transwell (n = 3) assays. Scale bar, 100 μm. Quantitative data are presented as mean ± SD of three replicates. *p < 0.05 and **p < 0.01, compared with the vector group; ^#p < 0.05, compared with the OTUB2 OE group.