TABLE 1.
Relative abundance of clones from triplicate unimproved and improved grassland soil samples and belonging to a number of bacterial phylogenetic groups
| Phylogenetic groupa | Relative clone abundance (%)
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Library from unimproved grassland
|
Library from improved grassland
|
Grassland mean value
|
Pooled data set (improved and unimproved grasslands) | ||||||
| SAF1 | SAF2 | SAF3 | SL1 | SL2 | SL3 | Unimproved | SL improved | ||
| Proteobacteria | |||||||||
| α-Proteobacteria | 52.1 | 24.4 | 53.3 | 22.2 | 43.5 | 37.0 | 43.3 (±18.5)b | 34.2 (±12.3) | 39.6 |
| β-Proteobacteria | 8.3 | 11.1 | 2.2 | 2.2 | 2.2 | 6.5 | 7.2 (±5.1) | 3.6 (±2.8) | 5.6 |
| γ-Proteobacteria | 2.1 | 0 | 4.4 | 6.7 | 2.2 | 6.5 | 2.2 (±2.5) | 5.1 (±2.9) | 3.7 |
| β-/γ-Proteobacteria | 4.2 | 11.1 | 4.4 | 0.0 | 2.2 | 0 | 6.6 (±4.4) | 0.7 (±1.4) | 3.7 |
| Flavobacteria | 0 | 2.2 | 0 | 17.8 | 0 | 4.3 | 0.7 (±1.5) | 7.4 (±10.5) | 4.1 |
| Green sulfur bacteria | 0 | 0 | 2.2 | 0 | 0 | 0 | 0.7 (±1.5) | 0 (ND)c | 0.4 |
| Actinomycetes | 2.1 | 17.8 | 4.4 | 13.3 | 19.6 | 22.2 | 8.1 (±9.6) | 18.2 (±4.9) | 13.3 |
| Low-G+C-content bacteria | 0 | 0 | 2.2 | 0 | 0 | 2.2 | 0.7 (±1.5) | 0.7 (±1.4) | 0.7 |
| Deinococcus-Thermus group | 0 | 0 | 2.2 | 0 | 0 | 0 | 0.7 (±1.5) | 0 (ND) | 0.4 |
| Acidimicrobium-Microthrix group | 2.1 | 15.6 | 6.7 | 6.7 | 8.7 | 0 | 8.1 (±7.8) | 5.1 (±5.1) | 6.7 |
| Rubrobacter | 6.3 | 4.4 | 0 | 11.1 | 4.3 | 2.2 | 3.6 (±3.6) | 5.9 (±5.3) | 4.8 |
| Holophaga | 4.2 | 8.9 | 0 | 4.4 | 4.3 | 0 | 4.4 (±5.0) | 2.9 (±2.9) | 3.7 |
| Acidobacterium | 4.2 | 0 | 0 | 6.7 | 4.3 | 2.2 | 1.4 (±2.7) | 4.4 (±2.5) | 3.0 |
| Verrucomicrobium | 0 | 2.2 | 6.7 | 4.4 | 0.0 | 4.3 | 3.0 (±3.8) | 2.9 (±2.9) | 3.0 |
| Unclassified groupsd | |||||||||
| Sourhope group 1 | 4.2 | 2.2 | 2.2 | 0 | 0 | 2.2 | 2.9 (±1.3) | 0.7 (±1.4) | 1.9 |
| Sourhope group 2 | 2.1 | 0 | 0 | 0 | 2.2 | 0 | 0.7 (±1.4) | 0.7 (±1.4) | 0.7 |
| Sourhope group 3 | 4.2 | 0 | 0 | 0 | 0 | 2.2 | 1.4 (±2.7) | 0.7 (±1.4) | 1.1 |
| Sourhope group 4 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 (ND) | 0.7 (±1.4) | 0.4 |
| Sourhope group 5 | 4.2 | 0 | 0 | 2.2 | 2.2 | 0 | 1.4 (±2.7) | 1.5 (±1.4) | 1.5 |
| Sourhope group 6 | 0 | 0 | 4.4 | 0 | 0 | 0 | 1.5 (±2.9) | 0 (ND) | 0.7 |
| Sourhope group 7 | 0 | 0 | 4.4 | 2.2 | 4.3 | 4.3 | 1.5 (±2.9) | 3.6 (±1.4) | 2.6 |
| Sourhope group 8 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 (ND) | 0.7 (±1.4) | 0.4 |
a
The numbers of clones in the libraries and data set were as follows: SAF1, 48; SAF2, 45; SAF3, 45; SL1, 45; SL2, 46; SL3, 46; and pooled data set, 275.
b
The values in parentheses are 95% confidence limits.
c
ND, not determined.
d
Unclassified Sourhope groups 1 through 8 are defined in the phylogenetic tree in Fig. 4.