Skip to main content
. 2022 Mar 30;21(5):e13593. doi: 10.1111/acel.13593

FIGURE 4.

FIGURE 4

Nav1.6 regulates BACE1 expression dependent on its channel property. Representative immunoblots (a) and densitometry analysis (b) of BACE1 and Nav1.6 expression levels in the HEK cell line overexpressing Nav1.6 and treated with TTX (1 μM). (c) Relative mRNA expression level of BACE1 in the HEK cell line overexpressing Nav1.6 after treatment with TTX (1 μM). Representative immunoblots (d) and densitometry analysis (e) of BACE1 and Nav1.6 expression levels in the HEK cell line overexpressing Nav1.6 in the presence of induced Aβ oligomers. (f) Relative mRNA expression level of BACE1 in the HEK‐APP cell line after treatment with different concentrations of TTX. Representative immunoblots (g) and densitometry analysis (h) of BACE1 and Nav1.6 expression levels in the HEK‐APP cell line transfected with NC or siNav1.6 plasmids with or without TTX (1 μM). Here, BACE1, APP, and Nav1.6 were normalized to γ‐tubulin in the Western blot, whereas BACE1 was normalized to β‐actin in the RT‐PCR. Data are presented as mean ± SEM (n = 5 biological replicates). *p < 0.05, ***p < 0.001