Table 1.
Primer sequences used in reverse transcription quantitative PCR (RT-qPCR)
| Gene | Species | Sequence (5′–3′) | |
|---|---|---|---|
| Lc3 | Mouse | Forward primer | CGTCCTGGACAAGACCAAGT |
| Reverse primer | ATTGCTGTCCCGAATGTCTC | ||
| Beclin | Mouse | Forward primer | AATCTAAGGAGTTGCCGTTATAC |
| Reverse primer | CCAGTGTCTTCAATCTTGCC | ||
| P62 | Mouse | Forward primer | GCTGCCCTATACCCACATCT |
| Reverse primer | CGCCTTCATCCGAGAAAC | ||
| Nos2 | Mouse | Forward primer | AATCTTGGAGCGAGTTGTGG |
| Reverse primer | CAGGAAGTAGGTGAGGGCTTG | ||
| Tnf-α | Mouse | Forward primer | TCGAGTGACAAGCCTGTAGCC |
| Reverse primer | TTGAGATCCATGCCGTTGG | ||
| Il-10 | Mouse | Forward primer | GCCAGAGCCACATGCTCCTA |
| Reverse primer | GTCCAGCTGGTCCTTTGTTTG | ||
| GAPDH | Mouse | Forward primer | TGGCCTTCCGTGTTCCTAC |
| Reverse primer | GAGTTGCTGTTGAAGTCGCA | ||
| CD68 | Human | Forward primer | GGAAATGCCACGGTTCATCCA |
| Reverse primer | TGGGGTTCAGTACAGAGATGC | ||
| Il31ra | Human | Forward primer | AACATAGCGAAAACTGAACCACC |
| Reverse primer | GCCAACTCAGGCTTTATCCATTC | ||
| Nppb | Human | Forward primer | TGGAAACGTCCGGGTTACAG |
| Reverse primer | CTGATCCGGTCCATCTTCCT | ||
| Trpv4 | Human | Forward primer | CTACGGCACCTATCGTCACC |
| Reverse primer | TTAGGCGTTTCTTGTGGGTCA | ||
| Trpv1 | Human | Forward primer | CTGCCCGACCATCACAGTC |
| Reverse primer | CTGCGATCATAGAGCCTGAGG | ||
| Lc3b | Human | Forward primer | GATGTCCGACTTATTCGAGAGC |
| Reverse primer | TTGAGCTGTAAGCGCCTTCTA | ||
| GAPDH | Human | Forward primer | GGAGCGAGATCCCTCCAAAAT |
| Reverse primer | GGCTGTTGTCATACTTCTCATGG | ||