Table 1.
Effect of Different Concentrations of Polysorbate 80 on Particle Size, Polydispersity Index, and Zeta Potential of Different NLC Placebos (Mean ± SD, n = 3)
| Formulation Composition (%w/v) | P1 | P2 | P3 | P4 | P5 | P6 | P7 | P8 |
|---|---|---|---|---|---|---|---|---|
| Glyceryl distearate | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 |
| Oleic acid | 1.0 | 1.0 | 1.0 | 1.0 | – | – | – | – |
| Castor oil | – | – | – | – | ||||
| Polysorbate 80 | 0.75 | 1.0 | 1.5 | 2.0 | 0.75 | 1.0 | 1.5 | 2.0 |
| Poloxamer 188 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Glycerin | 2.25 | 2.25 | 2.25 | 2.25 | 2.25 | 2.25 | 2.25 | 2.25 |
| Water up to (mL) | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| Particle size (nm) | 384.4±7.9 | 291.6±10.4 | 211.7±4.5 | 142.3±3.9 | 420.2±12.2 | 352.5±5.4 | 261.3±6.5 | 225.2±10.2 |
| Polydispersity index | 0.41±0.06 | 0.42±0.08 | 0.39±0.02 | 0.38±0.01 | 0.55±0.07 | 0.55±0.02 | 0.59±0.03 | 0.57±0.09 |
| Zeta potential (mV) | −28.5±1.5 | −29.6±0.7 | −31.4 ±1.3 | −27.1±1.7 | −29.2±1.5 | −27.4±1.7 | −32.3±1.3 | −31.6±1.1 |
Abbreviation: P, placebo.