FIG. 6.

Inoculation of mixed surfaces. Bioluminescent E. coli O157:H7 strain L-2 was inoculated (9.2 × 10⁷ total CFU) onto carcass surface tissues that included lean and adipose surfaces across the tissue sections. (Images i) Prewash grayscale. (Images ii) Prewash bioluminescence superimposed on grayscale. (Images iii) Bioluminescence superimposed on grayscale after a single wash. (Image iv) Bioluminescence after a second wash. A transitional tissue section with the overlying fascia tissue intact (a) and a transitional tissue section with the overlying fascia tissue removed by scalpel trimming (b) (indicated by the box in panel b, image i) were inoculated. In panel b inoculation included the section that had intact fascia and the section from which the fascia was removed (indicated by the box in image i). The arrows in panel a indicate the line of tissue topography transition. The arrows in panel b indicate the excision line between the intact fascia and the excised fascia.