TABLE 1.
Probe sequence used for FISH
Target | Sequence (5′-3′)a | Probe nameb | Reference |
---|---|---|---|
Control (no group)c | CCTAGTGACGCCGTCGAC | Control | 32 |
Universal | GWATTACCGCGGCKGCTG | S-*-Univ-0519-a-A-18 | 24a |
Bacteria | ACCGCTTGTGCGGGCCC | S-Bact-0927-b-A-17 | 18 |
α-Proteobacteria | CGTTCGYTCTGAGCCAG | S-Sc-aProt-0019-a-A-17 | 29 |
Cytophaga-Flavobacterium group | TGGTCCGTRTCTCAGTAC | S-*-CF-0319-a-A-18 | 3 |
W = A or T; Y = C or T; R = A or G.
Probe names based on names in the Oligonucleotide Probe Database (31a).
There were more than three mismatches compared with all of the rRNA sequences in the RDP database, and there was no appropriate Oligonucleotide Probe Database name.