Figure 3.

Increased atherosclerosis in Apoe^−/−/Hmgcr KI is associated with increased Ly6-C^hi monocytosis. (A-E) Apoe^−/− and Apoe^−/−/Hmgcr KI mice were aged to 20 weeks old on a standard chow diet and blood were collected at the end of the study. Circulating leukocytes were quantified by flow cytometry. (C) Ly6-C^hi monocytes, (D) Ly6-C^lo monocytes and (E) neutrophil populations were identified and overlayed on the same FACS plot for visualization purpose. Results are means ± SEM and analysed using a Student unpaired t test, n = 8 per group (∗P < 0.05 when comparing Apoe^−/− versus Apoe^−/−/Hmgcr KI).