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. 2022 May 23;158(3):241–251. doi: 10.1007/s00418-022-02115-y

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — IBV-induced ERC compaction and Golgi fragmentation follow similar kinetics. a, b Representative CM images of cells fixed at 12 (a) or 9 hpi (b), and double-stained for the viral N protein and Rab11 and ERGIC-53 to localize REs/ERC or cis-Golgi elements, respectively. The nuclei were visualized by DAPI staining. Both Rab11 and ERGIC-53 display clearly distinct localization patterns in the infected and uninfected (asterisks) cells, due to ERC compaction (a) or fragmentation of the Golgi ribbon (b). Also note variable positioning of the compact ERCs in IBV-infected cells (a, open arrowheads), based on centrosome motility. Besides cis-side of the Golgi ribbon, ERGIC-53 also localizes to peripheral IC elements (b, white arrowheads). c) Quantitation of cells showing ERC compaction (Rab11) or Golgi fragmentation (ERGIC-53 or GM130) in the course of IBV infection. d) A merged CM image of cells fixed at 9 hpi and double-stained for Rab11 and the Golgi marker GalNT2 to identify and quantitate cells showing both ERC compaction (open arrowheads) and Golgi fragmentation (white arrowheads). Bars, 5 μm