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. 2022 May 9;13:897046. doi: 10.3389/fphar.2022.897046

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Copyright © 2022 Zheng, Wang, Chen, Chen, Wu, Hu and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of FUNDC1 in podocytes suppressed the mitophagy induced by PP2. (A) Representative Western blot images of FUNDC1, p-FUNDC, LC3, and P62 expression in podocytes after the indicated treatments. (B–E) Quantification of Western blot band intensities of the different proteins; GAPDH was used as loading control. (F) Double immunofluorescence staining of LC3 (red) and TOMM20 (green) in podocytes after the indicated treatments (Magnification, 400 ×). Values are expressed as the mean ± SD, *p < 0.05 vs. HG group, ^# p < 0.05 vs. HG + Control siRNA group, ^@ p < 0.05 vs. HG + PP2 group.