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. 2022 May 9;9:900978. doi: 10.3389/fcvm.2022.900978

Figure 5.

Figure 5

Transcription regulatory network analysis uncovers Jun as a regulator of vascular aging. (A) Heatmap of transcription factor (TF) activities at six time points. (B) Gene network showing target genes of Jun. The dot size presents relative weight values in the regulatory network. (C) Lollipop chart showing enriched GO pathways of Jun target genes. (D) qRT-PCR analysis of Jun mRNA levels in young (3 m) vs. aged mouse (22 m) aorta. Data are presented as the mean ± SEM (n = 3) biological repeats. *p < 0.05 (Student's t-test). (E) SA-β-gal staining in HUVECs treated with vehicle (Ctrl) or Dox (doxorubicin). Data are presented as the mean ± SEM (n = 3 independent experiments). *p < 0.05 (Student's t-test). Scale bar, 50 μm. (F) qRT-PCR analysis of mRNA levels of aging markers in HUVECs upon Dox treatment. Data are presented as the mean ± SEM (n = 3 independent experiments). *p < 0.05, **p < 0.01 (Student's t-test). (G) qRT-PCR analysis of JUN mRNA levels in HUVECs upon Dox treatment. Data are presented as the mean ± SEM (n = 3 independent experiments). *p < 0.05 (Student's t-test).