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. 2022 May 9;10:900777. doi: 10.3389/fcell.2022.900777

FIGURE 1.

FIGURE 1

Non-secretion of mutant MYOC-induced ER stress. (A) Immunofluorescence staining of LAMA 4 (red) and TIMP3 (Green) and phase contrast images in HTM cells.(B) Western blot detection of MYOC and GAPDH with or without dexamethasone treatment. (C) Bands intensity analysis in (B) using ImageJ software. ***, p < 0.001. (D) Western blot analysis of MYOC in culture medium of HTM cells overexpressing MYOC-WT or MYOC-N450Y (E). Immunofluorescence staining of MYOC (red) and KDEL (green) in HTM cells overexpressing MYOC-WT or MYOC-N450Y. (F) Western blot detection of MYOC, Grp78, Grp94 and GAPDH in HTM cells overexpressing MYOC-WT or MYOC-N450Y (G). Band intensity analysis in (F) using ImageJ software. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Empty: empty vector lentiviruses; MYOC-WT: lentiviruses expressing wild type myocilin gene; MYOC-N450Y: Lentiviruses expressing N450Y mutant myocilin gene.