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. 2022 May 9;10:900777. doi: 10.3389/fcell.2022.900777

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Copyright © 2022 Yan, Wu, Liu, Cheng, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Autophagy inhibition rescues HTM cell viability and apoptosis (A) Western blot detection of P62, LC3B and GAPDH with or without chloroquine treatment. (B,C) Band intensity analysis in (A) using ImageJ software. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, no significance. (D) Cell count of HTM cells with or without chloroquine treatment. **, p < 0.01. (E) Cell viability was detected using CCK8 in HTM cells with or without chloroquine treatment. (F) Flow cytometry analysis of apoptosis of HTM cells in different groups. (G) Apoptosis ratio analysis of HTM cells in different groups. ns, no significance; **, p < 0.01; ***, p < 0.001. Empty: empty vector lentiviruses; MYOC-WT: lentiviruses expressing wild type myocilin gene; MYOC-N450Y: Lentiviruses expressing N450Y mutant myocilin gene.