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. 2022 May 9;10:900777. doi: 10.3389/fcell.2022.900777

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Copyright © 2022 Yan, Wu, Liu, Cheng, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mutant MYOC protein was expressed in the anterior angle of Tg-MYOC^N450Y mice (A) Schematic of Tg-MYOCN450Y construct. (B) PCR was applied to detect the expression of transgene MYOC-N450Y in Tg-MYOCN450Y mice at age of 5 months. (C) The presence of MYOC-N450Y was confirmed by sequencing. (D) Western blot was used to analyse the MYOC expression in anterior chamber angle tissue in WT littermates and Tg-MYOC^N450Y mice at 5 months of age. (E) Band intensity analysis in (D) using ImageJ software. *, p < 0.05. (F) Western blot was used to analysis MYOC secretion in aqueous humor. ns, no significance; *, p < 0.05. (G) Band intensity analysis in (F) using ImageJ software. *, p < 0.05. (H) Western blot was used to analyse the expression of Grp78, Grp94, LC3B, p62 and GAPDH at 8 months of age in WT littermates and Tg-MYOCN450Y mice.