FIG. 2.

Analysis by RT-PCR of clpg2 expression in planta. Total RNA extracted from bean hypocotyl epidermis 24 h after inoculation of the susceptible cultivar Early Wax with C. lindemuthianum race β (lanes 1) or from the corresponding healthy plant (lanes H) was used for RT-PCR analysis. To differentiate between genomic and mRNA-derived fragments, PCRs were done by using cloned cDNA (lane 2) and cloned genomic DNA (lane 3) corresponding to clpg2. The PCR products were analyzed by gel electrophoresis followed by ethidium bromide staining (panel A) and by Southern blotting by using a clpg2 probe (panel B). DNA size markers (100-bp ladder) were loaded on lane M. The sizes of two bands of the ladder, expressed in kilobase pairs, are indicated on the left.