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Orthodontic tooth movement (OTM) increases both Axin2+ progeny cell numbers and new bone formation. (A) Pentachrome staining images revealed changes in alveolar bone areas during OTM (immature matrix in green, red asterisks). (B) Colocalization of Axin2+ red progeny cells with RUNX2 immunostaining (green, white arrows). (C) Colocalization of Axin2+ red progeny cells with DMP1 immunostaining (green, white arrows). AB, alveolar bone; CONT, control; D, dentin; PDL, periodontal ligament.
