FIGURE 2.

Endothelial cell-specific deletion of β-catenin increases BBB permeability in adult mice. (A,C) Immunofluorescence staining showing leakages of blood IgG (A) or albumin (C) in the cerebral cortex from β-cat WT mice and β-cat ECKO mice, respectively. Scale bars, 100 μm. (B,D) Quantifications of the fluorescence intensity of IgG (B) and albumin (D) normalized with CD31 area, respectively. (E) Protein levels of IgG and albumin in the cerebral cortex from β-cat WT mice and β-cat ECKO mice. (F) Quantification of the IgG and albumin protein levels normalized with that of GAPDH. Data are mean ± SD. n = 4–5 mice per group. Significance of data is represented as: *P < 0.05, ^**P < 0.01, ^***P < 0.001.