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. 2022 May 9;9:870009. doi: 10.3389/fvets.2022.870009

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Copyright © 2022 Fang, Yang, Xu, Li, Li, Zheng, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ELISA was used to detect anti-p32 IgG in each group. Cut off calculation: cut off = average value of negative control + 0.15. When the negative control value was ≤ 0.05, it was calculated as 0.05. Positive judgment: samples with OD value ≥ cut off were judged as positive. Negative determination: samples with OD value < cut off were judged as negative. SFV-p32 RP-immunized mice are numbered 1–5, which are consistent with the numbers assigned to mice detected by pre-immune serum, the second immunization and the third immunization in the FIGURE.