FIGURE 3.

Characterization of inflammatory factors in Acinetobacter baumannii culture filtrate. (A,B) Culture filtrate from wildtype A. baumannii (Ab19606) was treated with DNase, RNase, proteinase K (Pro-K) or heat treated. THP1-XBlue reporter cells were exposed to untreated and the treated culture filtrate. Levels of SEAP were assessed after 24 h of culture filtrate treatment. The experiments were done in triplicates. Error bars represent standard deviation. One-way ANOVA with Tukey’s multiple comparisons test. (C) Organic extraction was performed on the culture filtrate from wildtype A. baumannii (Ab19606). THP1-XBlue reporter cells were treated with the Aqueous and organic phases. TSB medium was used to grow the bacterial cultures and fresh TSB medium treatment served as the negative control. Levels of SEAP were assessed after 24 h from the beginning of the treatment. The experiments were done in triplicates. Error bars represent standard deviation. One-way ANOVA with Tukey’s multiple comparisons test. (D) Biochemical fractionation was performed on the culture filtrate from wildtype A. baumannii (Ab19606). THP1-XBlue reporter cells were treated with the indicated fractions. Levels of SEAP were assessed after 24 h from the beginning of the treatment. The experiments were done in triplicates. Error bars represent standard deviation. One-way ANOVA with Tukey’s multiple comparisons test. All the experiments were performed three times independently. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.