FIGURE 8.

Gene prediction, maize germination, and toxicity assessment. (A) Prediction of genes related to siderophore synthesis in AS19. The arrow direction indicates the direction of transcription. The overlapped of arrows indicates the base sequence of overlap. (B) The germination of maize seeds treated with CFS and the bacterial solution of AS19. The SA medium group was the control group. (C) The germination percentage of maize seeds (a total of seven in each group). The experiment was repeated three times. Three dots represent the germination percentage of three experiments (ns, no significant difference, *p < 0.05). (D) Toxicity test of Hermetia illucens L. larvae. Improved survival rate of H. illucens L. larvae with CFS. H. illucens L. larvae were randomly divided into five groups. The natural growth (NG) group was only punctured. The control (Control) group was injected with 2.5 μL of sterile saline. The experimental group was injected with 2.5 μL of CFS (30 mg/kg, 40 mg/kg, and 1 g/kg). The number of surviving larvae was observed every 24 h, for 7 days. The survival rates were calculated, and the graph was drawn with GraphPad Prism 8.4 software (ns, no significant difference, *p < 0.05, n = 50).