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. 2022 May 9;13:842500. doi: 10.3389/fimmu.2022.842500

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Copyright © 2022 Peng, Sun, Zhang, Yan, Shi, Xia, Dai and Tang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-25 and HDM co-promote expression of surface HLA-DR, PD-L1, and OX-40L on eosinophils. (A) FACS plots of the purity for eosinophils (CD16^-Siglec^-8⁺ cells) after sorting. (B) Gated on viable eosinophils (Zombie^-CD16^-Siglec-8⁺ cells), expression rates of CD40, HLA-DR, CD80, CD86, PD-L1, and OX-40L in the presence or absence of HDM and/or IL-25 were tested by flow cytometry. (C) Graphs showed expression rates of CD40, HLA-DR, CD80, CD86, PD-L1, and OX-40L on eosinophils from allergic asthma patients. n = 14, ns, no significant difference, ^**p < 0.01, ^*p < 0.05.