Table 1.

After filtering the original data, checking the sequencing error rate, and checking the GC content distribution, we obtain clean reads for subsequent analysis. The data is summarized as shown in the table below.

Sample	Library	Raw_reads	clean_reads	Clean_bases	Error_rate	Q20	Q30	GC_pct
tVaS1	FRAS202156226-1r	43614846	42016796	6.3G	0.03	97.94	93.92	37.1
tVaS2	FRAS202156227-1r	45933166	44134864	6.62G	0.03	97.58	93.11	37.7
tVaS3	FRAS202156228-1r	45061154	43428666	6.51G	0.02	98.07	94.26	37.71
tVaR1	FRAS202156223-1r	43504158	42181696	6.33G	0.03	97.97	93.96	35.83
tVaR2	FRAS202156224-1r	44238968	42270438	6.34G	0.03	97.72	93.37	37.54
tVaR3	FRAS202156225-1r	46739216	43630738	6.54G	0.03	97.79	93.57	37.48
tCon1	FRAS202156229-1r	44446884	42744414	6.41G	0.02	98.04	94.16	38.45
tCon2	FRAS202156230-1r	45099750	43562032	6.53G	0.03	97.95	94	37.95
tCon3	FRAS202156231-1r	47643512	45841434	6.88G	0.03	98.01	94.12	38.14

Sample: Sample name.

Library: Library number.

Raw_reads: The number of reads in the raw data.

Clean_reads: The number of reads after filtering the original data.

Clean_bases: The number of bases after filtering the original data (clean base=clean reads*150bp).

Error_rate: The overall sequencing error rate of the data.

Q20: The percentage of bases with a Phred value greater than 20 to the total bases.

Q30: The percentage of bases with a Phred value greater than 30 to the total bases.

GC_pct: the percentage of G and C in the four bases in clean reads.