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. 2022 May 9;9:888360. doi: 10.3389/fnut.2022.888360

FIGURE 3.

FIGURE 3

Osteogenic activity of aqueous (A) and hydroethanolic (B) extracts from three species of sea cucumbers in zebrafish larvae. Results are displayed as corrected operculum area (operculum area/head ratio) expressed as percentage of increase over the control. Representative image (C) of a fish treated with the negative control (ethanol), the positive control (Calcitriol 10 pg/mL) and the most powerful osteogenic extracts among holothurians (H. mammata HE200). Statistical differences among the means were tested through one-way ANOVA followed by Dunnett’s multiple comparison test (p < 0.05) or, whenever normality and homoscedasticity weren’t met, through a non-parametric test followed by Dunn’s multiple comparison test (p < 0.05). P-values are indicated as follow: 0.0332 (*), 0.0002 (***), < 0.0001 (****). HE, hydroethanolic extracts, AQ, aqueous extracts, 100–100 μg/mL, 200–200 μg/mL.