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. 2022 Jan 25;34(4):1375–1395. doi: 10.1093/plcell/koac017

Figure 7.

Figure 7

ANAC017-dependent signaling is triggered by DTT and is required to mediate DTT tolerance of root growth. A, AOX1a transcript quantification by RT-qPCR from roots of 12-day-old Arabidopsis seedlings submerged in treatment solutions without (control) or with 2 mM DTT or 50 μM AA for 2 h. Lines: WT Col-0, rao2.1, anac017-1. AOX1a expression level relative to reference transcript TIP41. Mean + sd. N = 3 plates, with pooled roots from 18 to 20 seedlings each. Significant differences to WT according to one-way ANOVA with Dunnett’s multiple comparisons test (nsP > 0.05, *P < 0.01, and ***P < 0.001). B, D, and F, Primary root length increase of WT, rao2.1 and anac017-1 seedlings. Seedlings were grown vertically on half-strength MS agar plates for 4 days, then transferred to fresh plates supplemented with the treatment chemicals. (B) Control; (D) 400 µM DTT; (F) 10 µM AA. Representative images of seedlings 4 days after transfer. Scale bars: 10 mm. C, E, and G, Primary root growth within 4 days after transfer on new plates. (C) N = 17–18, (E) N = 18, (G) N = 17–18. Boxplot: first and third quartiles with median and Tukey’s whiskers. Differences were tested after log-transformation of data to establish normal distribution by one-way ANOVA with Bonferroni’s multiple comparisons test (nsP > 0.05, ***P < 0.001). P-values: Supplemental Data Set 7.