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. 2022 May 14;2022:1260423. doi: 10.1155/2022/1260423

Figure 6.

Figure 6

Overexpression of TP53INP2 resulted in an inhibition to cellular proliferation in ccRCC cell lines. (a) TP53INP2 overexpression was stably transfected into the 786-O, ACHN, and A498 ccRCC cell lines. The relative mRNA level of TP53INP2 was determined with qRT-PCR. (b) and (c) The level of TP53INP2 protein was determined with western blot. Relative values were estimated with Image J. (d)–(f) CCK-8 assay was made for the detection on the cell viability of 786-O, ACHN, and A498. (g)–(j) The flow cytometry was performed for the detection on the cell viability of 786-O, ACHN, and A498. Data were presented as mean ± SD of three or four independent experiments. ∗∗P < 0.01; ∗∗∗P < 0.001.