N. gonorrhoeae culture recovery decreases overtime. Culture recovery was calculated for urethral, endocervical, vaginal, rectal, and pharyngeal culture specimens collected on the same day (0), 1 to 7 days, 8 to 14 days, or ≥15 days after the NAAT specimen. Statistical differences between time points were determined by Fisher exact test or Chi-square test with Yates correction where applicable. ***P < 0.0001; **P < 0.01; *P ≤ 0.05.