In vivo uptake of PS 5 at the dose of 0.47μmol/kg in tumor, liver and skin of SCID mice bearing (A) UMUC3 tumors and (B) FaDu tumors. The in vivo PDT efficacy (long-term tumor response) of PS 5 at a dose of 0.47 μmole/kg for UMUC3 and FADU tumors are shown in (C) and (D) respectively. The tumors were exposed to light (665 nm, 135J/cm2, 75 mW/cm2) at 24 h post-injection of the PS. A direct correlation between the PS uptake and tumor response (cure) was observed. For PS-uptake study (tumor, liver and skin), 3 female SCID mice bearing either UMUC3 or FaDu tumors were used (Figures 11A & 11B). For determining in-vivo efficacy the number of female SCID mice used in each study are indicated in the Figure 9C & 9D. Statistical analysis log rank (Mantel-Cox test), (C):
p-value: <0.0046 and (D):
p-value: 0.0153.