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. Author manuscript; available in PMC: 2022 May 23.
Published in final edited form as: J Med Chem. 2021 Jan 5;64(1):741–767. doi: 10.1021/acs.jmedchem.0c01735

Figure 2.

Figure 2

Uptake and retention by HNT1 cells (passage 116). Confluent cell cultures were incubated for 30 min in serum-free medium containing 3.2 μM of the indicated PS. The cellular level and subcellular distribution of PS fluorescence indicates the binding to and mode of uptake by the cells. Subsequent treatment of the cells for 5 h with serum and PS-containing medium determines the competing effect of serum proteins on uptake and steady-state accumulation of the PSs. Follow-up incubation for 15 h in serum-containing medium but without PS defines the rate of egress of PS from cells. PS fluorescence is recorded at 100X magnification by different-length exposure of the camera. Images allow digital quantification of PS fluorescence level.