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Five-day old cocultures of HN-143 T-EC with HN-166 T-Fb were incubated with medium containing 10% FBS and 3 mM indicated PS for 5 h and then chased for 40 h with medium without PS. Phase contrast images at 100X magnification (upper panel in each section) and corresponding fluorescent images of all cultures (500 ms exposure time) were taken at the indicated time points (lower point in each section).
