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. Author manuscript; available in PMC: 2022 May 23.
Published in final edited form as: Obesity (Silver Spring). 2010 Oct 7;19(4):784–791. doi: 10.1038/oby.2010.223

Figure 6.

Figure 6

Lipolysis in white adipose tissue was similar in male transgenic mice and littermate control. (a,b) Basal and stimulated (+100 nmol/l isoproterenol) in vitro lipolysis in isolated epididymal adipose tissue from male transgenic mice and littermate control on LF and HF diet for 16 weeks (n = 4 mice/group). (c) The protein levels of total HSL, phospho-HSL, and PKG-I in the homogenates of epididymal fat from different groups of mice were determined by immunoblotting. β-Actin was used as an internal control. Representative blots of three independent experiments were shown. Results are the means ± s.e. *P < 0.05 vs. WT. #P < 0.05 vs. Tg (LF). FFA, free fatty acid; HF, high fat; HSL, hormone-sensitive lipase; LF, low fat; PKG-I, cyclic guanosine monophosphate–dependent protein kinase I; Tg, transgenic mice; WT, wild type.