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. 2022 May 19;10(5):e004834. doi: 10.1136/jitc-2022-004834

Figure 3.

Figure 3

9464D-GD2 neuroblastoma cells do not express MHC-I in the absence or presence of stimulation by IFN-γ. (A–B) MHC-I H-2Kb and H-2Db expression in parental 9464D (A) and 9464D-GD2 (B) cells in vitro as assessed by flow cytometry with or without IFN-γ. (C) Quantification of quantitative reverse transcription PCR analysis of MHC-I expression machinery in parental 9464D and 9464D-GD2 cells, with and without 48-hour stimulation with IFN-γ. All experiments performed in duplicate and a representative single experiment is shown. IFN, interferon; MHC-I, major histocompatibility complex class I.