Table 4.

Sources of NK cells and their unique advantages and disadvantages

Source	Advantages	disadvantages
PB	Mature phenotype Highly functional and cytotoxic	Only 5%–10% of PB lymphocytes are NK cells Heterogenous product Not readily available, need donors
CB	Readily available from global CB banks.15%–30% of CB lymphocytes are NK cells. Transcriptomic profile supports high proliferative potential	Numerically few and therefore requires ex vivo expansion Heterogeneous product
iPSC	High proliferative capacity Homogeneous product	Immature phenotype Low ADCC due to low CD16 expression Long culture condition
NK-92 cell line	High proliferative capacity Easy to manipulate and engineer Homogeneous product Reduced sensitivity to freeze/thaw cycles	Derived from a patient with NK lymphoma Need for irradiation Limited in vivo persistence following irradiation Low ADCC due to low or absent CD16 expression