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. 2022 Apr 11;322(6):E480–E493. doi: 10.1152/ajpendo.00002.2022

Table 3.

Indirect calorimetry analyses of male and female Ctrp14 KO mice and WT littermates fed a high-fat diet for 14 wk

WT KO P WT KO P WT KO P
Male, high-fat diet Ad libitum, dark cycle Ad libitum, light cycle Ad libitum, 24 h
 n 11 12   11 12   11 12  
 Body weight, g       40.46 ± 1.80 42.64 ± 1.66 0.384
 Food intake, kcal 11.77 ± 0.81 11.15 ± 0.55 0.544 4.29 ± 0.20 4.63 ± 0.18 0.257 15.45 ± 0.83 15.78 ± 0.58 0.740
 V̇o2, mL/kgLM/h 4,858 ± 88 4,807 ± 93 0.696 4,014 ± 68 4,075 ± 82 0.579 4,436 ± 74 4,441 ± 82 0.968
 V̇co2, mL/kgLM/h 3,998 ± 87 3,947 ± 65 0.642 3,285 ± 61 3,335 ± 68 0.593 3,642 ± 72 3,641 ± 62 0.994
 RER, V̇co2/V̇o2 0.822 ± 0.005 0.821 ± 0.003 0.922 0.817 ± 0.003 0.818 ± 0.002 0.843 0.819 ± 0.003 0.820 ± 0.002 0.982
 EE, kcal/kgLM/h 23.46 ± 0.44 23.20 ± 0.43 0.682 19.36 ± 0.33 19.65 ± 0.39 0.581 21.41 ± 0.37 21.42 ± 0.39 0.976
 Total activity, beam breaks 22,273 ± 1,937 20,462 ± 1,272 0.435 8,436 ± 910 6,767 ± 506 0.116 30,709 ± 2,806 27,230 ± 1,606 0.284
 Ambulatory activity, counts 11,370 ± 1,392 8,278 ± 751 0.058 3,639 ± 675 1,914 ± 228 0.020 15,009 ± 2,036 10,192 ± 921 0.037
Fasting, dark cycle Fasting, light cycle Fasting, 24 h
 Body weight, g     38.68 ± 1.69 39.82 ± 1.55 0.626
 Food intake, kcal 0 0 0 0 0 0
 V̇o2, mL/kgLM/h 4,416 ± 83 4,446 ± 92 0.822 3,438 ± 64 3,471 ± 70 0.746 3,931 ± 65 3,955 ± 80 0.824
 V̇co2, mL/kgLM/h 3,395 ± 64 3,427 ± 71 0.750 2,658 ± 47 2,678 ± 58 0.808 3,029 ± 50 3,050 ± 63 0.808
 RER, V̇co2/V̇o2 0.768 ± 0.001 0.770 ± 0.001 0.509 0.773 ± 0.001 0.771 ± 0.001 0.423 0.771 ± 0.001 0.771 ± 0.001 0.978
 EE, kcal/kgLM/h 21.03 ± 0.39 21.18 ± 0.44 0.807 16.39 ± 0.30 16.54 ± 0.34 0.759 18.73 ± 0.31 18.84 ± 0.38 0.821
 Total activity, beam breaks 31,377 ± 2,159 28,022 ± 1,372 0.203 7,652 ± 578 7,877 ± 623 0.800 39,029 ± 2,603 35,900 ± 1,606 0.316
 Ambulatory activity, counts 19,455 ± 1,422 14,899 ± 1,092 0.020 3,571 ± 328 3,148 ± 377 0.425 23,026 ± 1,656 18,047 ± 1,255 0.027
Refeed, dark cycle Refeed, light cycle Refeed, 24 h
 Body weight, g     39.99 ± 1.67 41.12 ± 1.57 0.631
 Food intake, kcal 17.09 ± 1.21 13.08 ± 0.69 0.010 3.95 ± 0.23 4.03 ± 0.28 0.849 20.46 ± 1.27 17.11 ± 0.84 0.041
 V̇o2, mL/kgLM/h 4,877 ± 85 4,805 ± 77 0.547 3,936 ± 63 3,941 ± 69 0.963 4,415 ± 72 4,381 ± 70 0.750
 V̇co2, mL/kgLM/h 3,958 ± 86 3,903 ± 53 0.596 3,299 ± 60 3,295 ± 52 0.957 3,634 ± 72 3,605 ± 50 0.743
 RER, V̇co2/V̇o2 0.811 ± 0.004 0.812 ± 0.004 0.796 0.838 ± 0.004 0.836 ± 0.003 0.719 0.824 ± 0.004 0.824 ± 0.003 0.972
 EE, kcal/kgLM/h 23.48 ± 0.43 23.14 ± 0.35 0.554 19.08 ± 0.31 19.09 ± 0.32 0.908 21.32 ± 0.36 21.15 ± 0.33 0.747
 Total activity, beam breaks 26,323 ± 1,271 25,459 ± 1,390 0.662 7,599 ± 530 7,041 ± 452 0.440 33,923 ± 1,562 32,500 ± 1,708 0.559
 Ambulatory activity, counts 14,272 ± 1,378 11,449 ± 863 0.096 3,450 ± 453 2,341 ± 260 0.045 17,722 ± 1,725 13,791 ± 1,044 0.064
Female, high-fat diet Ad libitum, dark cycle Ad libitum, light cycle Ad libitum, 24 h
 n 10 8   10 8   10 8  
 Body weight, g     31.51 ± 1.13 31.38 ± 1.21 0.938
 Food intake, kcal 9.23 ± 0.79 11.27 ± 1.44 0.210 2.61 ± 0.32 2.85 ± 0.24 0.577 11.85 ± 0.87 14.13 ± 1.42 0.174
 V̇o2, mL/kgLM/h 5,769 ± 126 5,613 ± 161 0.451 4,956 ± 140 4,697 ± 139 0.215 5,363 ± 131 5,155 ± 149 0.310
 V̇co2, mL/kgLM/h 4,765 ± 114 4,683 ± 140 0.653 4,039 ± 107 3,866 ± 113 0.288 4,402 ± 107 4,275 ± 125 0.450
 RER, V̇co2/V̇o2 0.825 ± 0.006 0.833 ± 0.005 0.383 0.814 ± 0.004 0.822 ± 0.002 0.162 0.819 ± 0.005 0.827 ± 0.003 0.244
 EE, kcal/kgLM/h 27.88 ± 0.618 27.18 ± 0.78 0.489 23.88 ± 0.668 22.68 ± 0.67 0.228 25.88 ± 0.62 24.93 ± 0.72 0.334
 Total activity, beam breaks 24,346 ± 3,097 31,071 ± 2,038 0.106 8,700 ± 842 10,086 ± 846 0.268 33,046 ± 3,831 41,157 ± 2,838 0.123
 Ambulatory activity, counts 11,669 ± 1,894 15,065 ± 1,234 0.175 2,895 ± 305 3,308 ± 367 0.395 14,565 ± 2,168 18,374 ± 1,559 0.193
Fasting, dark cycle Fasting, light cycle Fasting, 24 h
 Body weight, g       24.15 ± 4.09 26.26 ± 3.90 0.718
 Food intake, kcal 0 0 0 0 0 0
 V̇o2, mL/kgLM/h 5,425 ± 157 5,158 ± 166 0.264 4,314 ± 161 4,110 ± 180 0.411 4,870 ± 156 4,634 ± 171 0.325
 V̇co2, mL/kgLM/h 4,181 ± 113 4,017 ± 127 0.350 3,336 ± 123 3,193 ± 146 0.461 3,759 ± 115 3,605 ± 135 0.397
 RER, V̇co2/V̇o2 0.770 ± 0.001 0.778 ± 0.001 0.002 0.773 ± 0.003 0.775 ± 0.002 0.565 0.771 ± 0.001 0.777 ± 0.001 0.061
 EE, kcal/kgLM/h 25.85 ± 0.74 24.62 ± 0.79 0.279 20.57 ± 0.76 19.61 ± 0.86 0.421 23.21 ± 0.73 22.12 ± 0.81 0.338
 Total activity, beam breaks 26,463 ± 4,383 35,042 ± 2,967 0.144 8,319 ± 1,077 8,997 ± 626 0.618 34,782 ± 5,281 44,039 ± 3,137 0.177
 Ambulatory activity, counts 14,195 ± 2,848 19,487 ± 2,025 0.169 3,107 ± 497 3,371 ± 343 0.683 17,302 ± 3,253 22,859 ± 2,034 0.192
Refeed, dark cycle Refeed, light cycle Refeed, 24 h
 Body weight, g       30.21 ± 1.10 30.11 ± 1.20 0.951
 Food intake, kcal 10.77 ± 0.62 11.77 ± 0.91 0.368 2.92 ± 0.35 3.34 ± 0.38 0.437 13.69 ± 0.62 15.11 ± 1.01 0.233
 V̇o2, mL/kgLM/h 5,587 ± 121 5,391 ± 195 0.388 4,849 ± 155 4,695 ± 133 0.476 5,226 ± 135 5,052 ± 161 0.418
 V̇co2, mL/kgLM/h 4,624 ± 90 4,536 ± 145 0.598 4,046 ± 126 3,968 ± 111 0.656 4,341 ± 106 4,259 ± 123 0.620
 RER, V̇co2/V̇o2 0.827 ± 0.005 0.842 ± 0.005 0.096 0.833 ± 0.005 0.844 ± 0.004 0.167 0.830 ± 0.005 0.843 ± 0.004 0.114
 EE, kcal/kgLM/h 27.01 ± 0.57 26.15 ± 0.92 0.423 23.48 ± 0.74 22.80 ± 0.64 0.510 25.28 ± 0.64 24.52 ± 0.76 0.453
 Total activity, beam breaks 23,413 ± 2,312 28,207 ± 1,264 0.110 8,933 ± 923 11,089 ± 1,712 0.258 3,2347 ± 2,971 39,297 ± 2,503 0.102
 Ambulatory activity, counts 10,531 ± 1,316 12,989 ± 767 0.151 2,961 ± 277 4,184 ± 959 0.196 13,492 ± 1,473 17,174 ± 1,386 0.093

All data are presented as means ± SE. All significant data (P < 0.05) are underlined and bolded. EE, energy expenditure; KO, knockout; RER, respiratory exchange ratio; V̇co2, rate of carbon dioxide production; V̇o2, rate of oxygen consumption; WT, wild type.