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. 2022 May 23;18(5):e1010209. doi: 10.1371/journal.pgen.1010209

Fig 3. Progressive loss of TTX-3 and CEH-10 expression in PRC1 mutants.

Fig 3

(A) Expression of the TTX-3 protein (ttx-3endo::yfp (vba3)) and the CEH-10 protein (ceh-10endo::yfp (vba1)) in embryos (epidermal enclosure for TTX-3, 1.5-fold for CEH-10) and early larvae. Ventral views, anterior is left, scale bar = 10 μm. (B) Percentage of animals that display a loss of TTX-3 protein (upper part) or CEH-10 protein (lower part) expression in at least one AIY neuron (error bars show standard error of proportion, numbers above the bars show number of animals analyzed). Expression in the embryo was analyzed at the stage when expression is initiated: epidermal enclosure for TTX-3 and 1.5-fold for CEH-10. For early larval (L1), late larval (L4) and young adult stages, TTX-3 and CEH-10 expressions were analyzed in parallel in the same animals using two color imaging. In most cases (86% of the neurons with a loss of expression) both TTX-3 and CEH-10 expression are lost. In the remaining cases (14%) only TTX-3 expression is lost while CEH-10 expression is still detectable. Statistical tests were performed between wild type and PRC1 mutants at each stage. They were also performed between embryonic and L1 larval stages, as well as between L1 larval and young adult stages in PRC1 mutants. A Fisher’s exact test was used (*** p<0.001, ** p<0.01, * p<0.05, ns not significant).