Table 1. Characteristics of microsatellite markers used in this study.
Locus | Primer sequence (5’-3’) | Motif | Ta(°C) | Dye | Allele size (bp) |
---|---|---|---|---|---|
Poolplexing group A | |||||
B3H | 5’-GGTATGCGTGGATATGAAGC-3′ | (AC)13 | 60 | 6-FAM | 59–93 |
5’-CTCGGCATCGCAGTTTC-3′ | |||||
AC52 | 5′-CATCTACGGCTGATGCAGAA-3′ | (CA)18TA(CA)14 | 58 | NED | 86–140 |
5′-CGTCTGGCTAAAGTGGGAAT-3′ | |||||
Ibh3 | 5′-GGAGAGGCTGCGATGTATCT-3′ | (GT)2GG (GT)2GG(GT)4 | 58 | HEX | 117–133 |
5′-CAGGGCTGTCTTGACGAAG-3′ | |||||
Poolplexing group B | |||||
E11 | 5′-TGCGTAGGGCAAAGGAGTT-3′ | (GA)10 | 58 | 6-FAM | 93–111 |
5′-GGGTGTCTGCCTGCATTC-3′ | |||||
7GN | 5’-CTCCTGGAACGGCTAACAC-3′ | (AC)11 | 60 | HEX | 105–131 |
5’-TGATATGAGGCACATTCAGC-3′ | |||||
LBA | 5′-CCTCTGTGAGAAGGCAAGGA-3′ | (GA)11 | 64 | HEX | 165–193 |
5’-GCTGCACATGCATTCTCTCGT-3′ | |||||
EMI | 5′-CGCTGAAGCACGGCGAATG-3′ | (GT)20 | 60 | NED | 180–206 |
5′-CGTAGCTCCTCTGTCCGTTC-3′ | |||||
Poolplexing group C | |||||
LRC | 5′-CTGCCTCTGCCTCACCTACT-3′ | (GT)17 | 60 | HEX | 112–166 |
5′-CTAACCCTCACACTCCCCATC-3′ | |||||
ARP | 5′-GGCTTCGGTCTGTTCGACTA-3′ | (GT)10 | 60 | NED | 129–173 |
5′-CACCCACTCGCATCCGTA-3′ | |||||
G09 | 5′-CAAGCAGGCAAGAGTCTGAAA-3′ | (CA)3(GA)12 | 60 | 6-FAM | 152–168 |
5′-GTCTCCCGTATTGCTCTCTCTA-3′ |
Ta: annealing temperature