FIG. 2.

Northern blot of RNA (5 or 10 μg per lane) prepared from sporulating cells (>80% phase-dull to phase-white endospores) hybridized with 35 pmol of a ³²P-labeled oligonucleotide specific for the cry1Ab gene (see Materials and Methods). Lane 1, 5 μg of RNA from B. thuringiensis subsp. aizawai HD133; lane 2, 5 μg of RNA from B. thuringiensis subsp. kurstaki HD1; lane 3, 10 μg of RNA from B. thuringiensis subsp. aizawai HD112; lane 4, 10 μg of RNA from B. thuringiensis subsp. tolworthi HD124.