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. 1999 May;65(5):1849–1853. doi: 10.1128/aem.65.5.1849-1853.1999

FIG. 3.

FIG. 3

Analysis of protoxins extracted from purified inclusions from B. thuringiensis subsp. kurstaki HD1 (lanes 1), B. thuringiensis subsp. aizawai HD133 (lanes 2), and (3) B. thuringiensis subsp. tolworthi HD124 (lanes 3). (A) Stained sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis gel with 2 μg of protein in each lane. The standards used (lane STD) were (from top to bottom) myosin (205 kDa), β-galactosidase (116 kDa), phosphorylase b (97.4 kDa), and bovine serum albumin (66 kDa). (B) Immunoblot obtained with a Cry1Ab monoclonal antibody containing the same amounts of protein. (C) Immunoblot obtained with a Cry1A polyclonal rabbit antibody.