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. 2022 May 16;2022:9613095. doi: 10.1155/2022/9613095

Figure 1.

Figure 1

Knockdown of HULC decreases viability and proliferation of HCC cells. (a) The transfection efficiency of si-HULC was verified with use of qRT-PCR. (b) Iron detection in BEL-7402 and SMMC-7721 cells transfected with si-HULC. (c) MTT was used to analyze BEL-7402 and SMMC-7721 cell viabilities transfected with si-NC or si-HULC. (d, e) Colony formation assay was used to assess cell proliferation. (f, g) Representative images and quantification of BEL-7402 and SMMC-7721 cell migration following HULC depletion in a scratch wound healing assay. Three independent experiments and sample size (n = 3), compared with the si-NC; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.