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. 2022 May 16;2022:9613095. doi: 10.1155/2022/9613095

Figure 6.

Figure 6

miR-3200-5p targeted ATF4. (a) The expression of ATF4 in HCC (n = 22) and normal liver (n = 17) tissues was detected with the use of immunohistochemistry. (b) The results of the double luciferase reporter gene showed that the gene ATF4 was associated with miR-3200-5p. (c, d) qRT-PCR was performed to detect the expression of ATF4 mRNA in HCC cells transfected with miR-3200-5p mimic/inhibitor. (e–h) Western blot was performed to detect ATF4 expression following the addition of the mimic miR-3200-5p, inhibitor miR-3200-5p, inhibitor miR-3200-5p+si-NC, or inhibitor miR-3200-5p+si-ATF4 in BEL-7402 and SMMC-7721 cells. Three independent experiments and sample size (n = 3), compared with the control group; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.