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. 2022 May 16;2022:8652741. doi: 10.1155/2022/8652741

Figure 3.

Figure 3

NBP inhibited oxidative stress injury and triggered nuclear translocation of Nrf2 and upregulated its downregulated genes. Effects of NBP on (a) SOD activity, (b) MDA content (n = 8 in each group); and (c) 8-iso PGF2α level (n = 10 in each group) after RCIR injury. (d)–(g) Representative immunoblots and densitometry analysis of nuclear-Nrf2, HO-1, and NQO1 in the hippocampus in four groups. (h)–(n) The mRNA levels of the Nrf2 and Nrf2 target genes in the hippocampus were evaluated. n = 6 in each group. (o) Localization of Nrf2 was performed by immunofluorescence staining in hippocampus 4 weeks after NBP treatment. Immunofluorescence labeling of Nrf2 (green) and nuclei was stained with DAPI (blue). The merged images showed the nuclear location of Nrf2 protein (400×, bar = 20 μm). n = 3 in each group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, the RCIR group vs. the sham group; #P < 0.05, ##P < 0.01, ###P < 0.001, the NBP80 group or the NBP120 group vs. the RCIR group; $P < 0.05, $$P < 0.01, the NBP80 group vs. NBP120 group. Values are expressed as the mean ± SD.