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IEF analysis of MnP separated from N. frowardii during SSF of wheat straw (lanes 1 and 2) and IEF analysis of MnP2 (lane 3) and MnP1 (lane 4) obtained from liquid cultures (51). MnP activity was stained with phenol red (lane 1) or ABTS (lanes 2 through 4) in the presence of Mn(II) and H2O2.
