Fig. 3.

Time-course for DOX release from prodrugs (10 μM) in ammonium bicarbonate buffer (0.1 M, pH 7.4) at room temperature and in the presence of H₂O₂ (10 μM). A calibration curve was used to determine the concentration of free DOX at each time point by LCMS. Plotted as the mean ± standard error of the mean (SEM) from three independent experiments.