Fig. 4.

Percentage of released DOX from c1 in response to various biological analytes during an 80 min incubation period at room temperature: (1) ammonium bicarbonate buffer (0.1 M, pH 7.5), (2) 10 μM H₂O₂, (3) 100 μM cysteine, (4) 100 μM homocysteine, (5) 1 mM glutathione, (6) 10 μM glutathione disulfide, (7) 10 μM sodium nitrite, (8) 10 μM sodium hypochlorite, (9) 10 μM superoxide, (10) 10 μM peroxynitrite. A calibration curve was used to determine the concentration of free DOX in response to each analyte. Plotted as the mean ± SEM from three independent experiments.