Fig. 6.

Uptake of DOX and prodrugs c1 and c2 by H9C2 cardiomyoblasts. H9C2 cells cultures grown overnight on chambered coverslips in media with 10% serum were switched to Fluorobrite DMEM imaging media supplemented with 5% serum and prepared for live-cell imaging on a Zeiss LSM 880 confocal microscope, then 10 μM of c1, c2, or DOX was added and images were taken every 10 min (averaged every 30 min) for 18 h. Approximately 20 cells present in each field of view were averaged for each sample and time point; normalized and averaged data from two replicates each ± SEM were included for c1 and DOX. An earlier independent trial yielded very similar results (Fig. S4).