Fig. 7.

Cytotoxicity of DOX and prodrugs in H9C2 cardiomyoblasts. Media from cells exposed for 24 or 48 h to 10 or 20 μM (A and B, respectively) of c1, c2, DOX or vehicle (DMSO, final concentration 0.1% in all samples) was centrifuged to remove cells and cell debris, and supernatants were assessed spectrophotometrically by lactose dehydrogenase (LDH) assay to evaluate release into the media as a measure of cytotoxicity (n = 6 or more). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.