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. 2022 May 23;2:56. doi: 10.1038/s43856-022-00113-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Direct linkage via cysteine groups of VHH-72-13C. B Maleimide-thiol based grafting onto a functional PEG₆ spacer. C C_1s high resolution XPS spectra of Au-VHH-72-C13 and D Au-PEG₆-VHH-72-C13 surfaces. E Differential pulse voltammograms (DPVs) of Au-VHH-72-C13 (black) and Au-PEG₆-VHH-72-C13 (grey) in ferrocenemethanol (1 mM in 0.1 M PBS, pH 7.4). F Au-PEG₆-MAL interface modified with 6-(ferrocenyl) hexanethiol and the corresponding cyclic voltammogram in PBS (0.1 M PBS, pH 7.4). Control: immobilization of ferrocene (grey line) scan rate: 100 mV s⁻¹.