Fig. 1.

Mitochondrial thioredoxin and thioredoxin reductase genes are specifically upregulated in long-lived mitochondrial mutants. To examine the role of thioredoxin and thioredoxin reductase in the longevity of long-lived mutants, we quantified gene expression from RNA sequencing data of six biological replicates per strain. Expression of the mitochondrial thioredoxin system was specifically upregulated in the long-lived mitochondrial mutants nuo-6, isp-1 and clk-1, but not in other non-mitochondrial, long-lived strains (A). Blue bars indicate long-lived mitochondrial mutants. Thioredoxin mRNA expression in long-lived mitochondrial mutants compared to wild-type (B). Thioredoxin reductase mRNA expression in long-lived mitochondrial mutants compared to wild-type (C). Statistical significance was assessed using a one-way ANOVA with Dunnett's multiple comparison test. Error bars indicate SEM. *p < 0.05, **p < 0.01, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)